Molecular identification and quantification of bacteria from endodontic infections using real-time polymerase chain reaction. A substantial expansion in knowledge of the diversity of the endodontic microorganisms involved with different clinical conditions has occurred over the last decade. This is because different species may apparently occupy the same niche in different communities as suggested by studies showing a high interindividual variability in the endodontic bacterial communities associated with the same disease condition 18 , So far, virtually all studies involved with phenotypic or genotypic identification of endodontic bacteria have followed a cross-sectional design, for obvious ethical reasons. Cultivable bacteria in infected root canals as identified by 16S rRNA gene sequencing. Thus, species prevalence and consequently only species association with disease can be inferred from these studies. Bacterial counts per abscess case have been reported to range from 10 4 to 10 9 colony forming units 46 , 71 ,
Based on cross-sectional studies, several species have emerged as candidate or putative endodontic pathogens, and the labels candidate or putative should be preserved while a definite role in disease causation is not determined. Therefore, any species found in the canal has the potential to be an endodontic pathogen or at least to play a role in the ecology of the microbial community. Two calibrated endodontists assessed outcomes blinded to preoperative status. Uncultivated phylotypes from several genera have been identified, including Dialister , Prevotella , Synergistes , Solobacterium , Olsenella , Fusobacterium , Eubacterium , Megasphaera , Veillonella , and Selenomonas , as well as phylotypes related to the family Lachnospiraceae or the TM7 phylum 22 , 25 — 28 , 65 — Different formats are available for download.
As perodontitis knowledge is refined and incorporated into the context of community as the unit of pathogenicity 12identification of different microbial community patterns associated with specific disease manifestations has the perildontitis to reveal idiosyncrasies that may be used as the foundation for the establishment of evidence-based antibacterial treatment protocols. Eur J Oral Sci. Herpesviruses in endodontic pathoses: After making a selection, click one of the export format buttons.
Metagenomic analysis of the human distal gut microbiome.
Actually, the structure of the microbiota may be responsible for the different clinical presentations of apical periodontitis. Molecular analysis of the root canal microbiota associated with endodontic treatment failures.
Other bacteria found in root canal-treated teeth with dizsertation periodontitis include Streptococcus species and some fastidious anaerobic species — P. Bacterial reduction in infected root canals treated with 2. Molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections.
Direct amplification of rRNA gene sequences for identification of selected oral pathogens in root canal infections.
Secondary infections are caused by microorganisms that were not present in the primary infection, but that were introduced in the root canal at some time after professional intervention. Advances in knowledge and technology open the perspectives for many questions to be answered and consequently many others to be raised in the near dissertatikn.
Distinctive features of the microbiota associated with different forms of apical periodontitis
Scand J Dent Res. Metadata Show full item record.
See yesterday’s most popular searches here. Different formats are available for download. Periodontitia infections develop in a previously sterile place which as such does not contain a normal microbiota.
Show downloadable dissertations only. As for the lesion specimens, bacteria were not detected in six cases Strengthening of the association apicaal apical periodontitis of some species previously recognized as candidate pathogens has also become evident by molecular findings.
Facultative gram-negative enteric rods in persistent periapical infections.
The Association of Statin Intake and Healing of Apical Periodontitis After Root Canal Treatment
The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. In that case, bacteria were found in the associated lesion.
Published online Aug Microorganisms from canals of root-filled teeth with periapical lesions. Search for dissertations about: On bacteria Persisting Root Canal Treament. Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment.
Fungi and most recently archaea and viruses have been found in association with endodontic infections 4 — 8but bacteria are unarguably the major microorganisms a;ical in the pathogenesis of apical periodontitis. In terms of bacterial density, a treated canal associated with post-treatment disease can harbor 10 3 peroodontitis 7 cells 39, Association of black-pigmented bacteria with endodontic infections.
Associations between microbial species in dental root canal infections. The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing. Molecular identification of microorganisms from endodontic infections.
Information peridoontitis by these methods has the potential to disclose patterns of molecules associated with diverse clinical conditions, helping establish outcome predictors based not only in specific species combinations or communities, but perhaps even more importantly on the resultant products of these consortia.
Sobkowiak ; Karolinska Institutet.